Catalase and hydrogen peroxide coursework

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

If you show you understand this though it shouldn't matter. I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks.

The muscle of patients undergoing haemodialysis undergoes some adaptive responses in total glutathione content, heat shock protein content and catalase activity that are potentially related to chronic oxidative stress.

HuGE Navigator Observational study of gene-disease association and gene-gene interaction. Whilst I'm sure your experiment won't contain such a gaping hole in it's method it will surely contain some weaknesses that you will need to show in your results and account for in your next section.

Tabulate all the values recorded in the step D. For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules.

The oxygen produced in 30 seconds is collected over water. A small sample of bacteria is collected on the end of the capillary tube, without blocking the tube, to avoid false negative results.

I have drawn a line of best fit to clearly illustrate this trend. Oxygen is soluble in water, but dissolves only slowly in water at normal room temperatures. A statistically significant higher malondialdehyde concentration in erythrocytes and blood plasma and a higher activity of SOD or CAT in erythrocytes was shown in patients with a brain tumour.

One of the best things to do here is not to just interpret the results from a single chart but draw one conclusion from the results of many. It may not be possible to i Source s: Gene-environment interactions support plausibility of CAT gene for the development of bronchial asthma.

Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Choice of Method I tried to select the method I considered would be most accurate. Read up on catalase as a catalyst in this reaction and what factors affect its efficacy.

No evidence for a major effect of CT or C T sinsgle nucleotide polymorphism on type 1 diabetes mellitus susceptibility in two large sample collections.

I did modular chemistry at school so didn't have to do any noncey experiments like this but I guess some of us aren't suited to just blowing stuff up at the back of the lab so have to go the Salters route. Sirt1 overexpression rescued H 2 O 2 -induced apoptosis through the upregulation of catalase.

Michaelis-Menton mechanism of enzyme catalysis. Obviously I would run these ideas by your teacher first as they will be more up on your curriculum and be able to confirm whether you should include them.

Microbiology - 008 - Catalase Test

Measure another 20 cm3 pureed potato into it. Then the rate of reaction is calculated. Although I tried to control the temperature in a water bath, and to good effect a constant external temperature was produced and the heat energy was dissipatedI could not control the amount of heat given off in each reaction.

This beetle has two sets of liquids that are stored separately in two paired glands. This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had used exactly 0. To prevent this I had to dry out the barrel and syringe before commencing the procedure.

Apparatus and Chemicals For each group of students: Surface Area of Yeast Molecules I ground up the yeast to try to make the surface area as similar as possible because surface area is a major factor in my experiment.

What results would you expect to see? In this procedure, 2 cm3 of 10 vol hydrogen peroxide will release 20 cm3 of oxygen if the reaction goes to completion.

Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously. For a given initial concentration of substrate, [S]0, the initial rate of product formation is proportional to the total concentration of enzyme [E]0.

This is shown in the table with all the results I have gained Fig. This heat energy is transferred to the environment. We have developed a novel AAV vector to enhance catalase expression. Furthermore, the poison cyanide is a noncompetitive inhibitor [20] of catalase at high concentrations of hydrogen peroxide.

How will this affect the results? Enhanced expression of the antioxidant enzyme catalase in human T cells can protect them against reactive oxygen species.Catalase performs its rapid destruction of hydrogen peroxide in two steps. First, a molecule of hydrogen peroxide binds and is broken apart. One oxygen atom is extracted and attached to the iron atom, and the rest is released as harmless water.

Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

It promotes the conversion of hydrogen peroxide, a powerful and potentially harmful oxidizing agent, to water and molecular oxygen.

Oct 27,  · Catalase and decomposition of hydrogen peroxide? i chose this experiment for my salters course simply because its meant to be easy. i know i can measure temperature concentration and ph or Resolved.

Investigation - Hydrogen Peroxide An investigation into how the concentration of the substrate 'hydrogen peroxide' affects the rate at which it is broken down to oxygen and water by the enzyme catalase.

Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals).It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS).

Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase. Class practical or demonstration Hydrogen peroxide (H2O2) is a by-product of respiration and is made in all living cells. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell.

Cells make the enzyme catalase to remove hydrogen peroxide.

Science Coursework. Line of best fit - hydrogen Peroxide and Catalase? Download
Catalase and hydrogen peroxide coursework
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